HIV-1 Standard

at the University of Maryland School of Medicine (UMSOM)

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Human Immunodeficiency Virus Type 1 (HIV-1) Quantitative RT-PCR (Standard)

Test Mnemonic HIV-1 Viral Load (Standard)
Detection HIV-1 Viral Load
Methodology Roche Amplicor Monitor Assay by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) followed by ELISA detection.
Service  Monday - Friday.
Turn Around Time 5 days.
Collection Tubes EDTA (lavender top) or ACD (yellow-top) 

Note: Heparinized samples cannot be used.

Specimen Requirements

Whole blood (min. 3 mL)  

Use standard procedures to remove plasma from cells within 3 hours of collection.  Specimen may be stored at 2-8°C for up to 5 days.

Special Instructions:  Store and transport at room temperature; Specimen must be received within 48 hours of collection; Plasma may be stored frozen at -20°C for 72 hours or -80°C indefinitely. 

Please complete the Diagnostic Service Request Form (shown below) and forward it with the specimen.

Rejection Criteria:  Heparinized specimen.  Plasma left on cells for >3 hours.

Reference Range 400-750,000 viral particles per mL
Contact tel: 410-706-0513; fax: 410-706-8623



Diagnostic Service Request Form

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Last updated: 05/10/13

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