VDJ-PCR

at the University of Maryland School of Medicine (UMSOM)

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Gene Arrangement of Immunoglobulin Heavy Chain (IgH) Variable (VH)-Diversity (DH)-Joining (JH) Region by PCR

Test Mnemonic VDJ-PCR
Detection Gene arrangement of VDJ region
Methodology PCR and gel electrophoresis
Service  Monday - Friday.
Turn Around Time 9 days.
Collection Tubes EDTA (lavender top) or ACD (yellow-top) 

Note: Heparinized samples cannot be used.
Specimen Requirements

Whole blood (min. 6 mL) /Bone marrow 2 mL/CSF 5-10 mL/Tissue biopsy approx. 200 mg and frozen within 1 hour of collection

Special Instructions:  Store and transport at room temperature; Specimen must be received within 48 hours of collection; Plasma may be stored frozen at -20°C for 72 hours or -80°C indefinitely. 

Please complete the Diagnostic Service Request Form (shown below) and forward it with the specimen.

Rejection Criteria:  Heparinized specimen.  Plasma left on cells for >3 hours.
Sensitivity 0.1-10%
Contact tel: 410-706-0513; fax: 410-706-8623

 

 

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Last updated: 04/18/10

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